D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80S

Expression Data

Yield (mg/mL): 2.312

Kinetic Assay Data

**slope of A80S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		6.07E-02	6.01E-02
B	25.00		4.19E-02	4.39E-02
C	8.33
D	2.78	3.49E-02	2.82E-02	2.94E-02
E	0.93	1.93E-02	2.43E-02	2.23E-02
F	0.31	9.20E-03	9.85E-03	9.65E-03
G	0.10	3.35E-03	5.80E-03	5.15E-03
H	0.03	0	0

Yield (mg/mL): 2.312 Dilution Factor: 100×

Protein purified on 7/16/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:24:08.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.76E-01	1.69E-01
B	25.00		1.33E-01	1.39E-01
C	8.33
D	2.78	1.03E-01	1.10E-01	1.13E-01
E	0.93		6.52E-02	6.21E-02
F	0.31	4.24E-02	3.34E-02	2.75E-02
G	0.10	1.80E-02	1.39E-02	1.02E-02
H	0.03	2.50E-04	2.50E-04

Yield (mg/mL): 2.14 Dilution Factor: 100×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:25:41.

Design Data

Rosetta Score for A80S: 2.704

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.