D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315E

Expression Data

Yield (mg/mL): 1.784

Kinetic Assay Data

**slope of H315E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.02E-01	1.04E-01
B	25.00		8.60E-02	8.02E-02
C	8.33	6.86E-02	6.49E-02	5.87E-02
D	2.78	5.09E-02	4.41E-02	6.20E-02
E	0.93	2.48E-02	2.76E-02	2.46E-02
F	0.31	1.25E-02	1.24E-02	1.06E-02
G	0.10	5.00E-03	4.80E-03	4.20E-03
H	0.03	2.50E-04	2.50E-04	2.50E-04

Yield (mg/mL): 1.784 Dilution Factor: 100×

Protein purified on 7/10/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:51:48.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.99E-01	3.02E-01
B	25.00		2.58E-01	2.59E-01
C	8.33		1.87E-01	1.97E-01
D	2.78	1.68E-01	1.39E-01	1.37E-01
E	0.93	9.86E-02	7.20E-02	7.67E-02
F	0.31	3.85E-02	3.43E-02	3.32E-02
G	0.10	1.70E-02	1.44E-02	1.20E-02
H	0.03	4.50E-04	3.00E-04	0

Yield (mg/mL): 4.71 Dilution Factor: 100×

Protein purified on 7/16/2024 and assayed on 7/18/2024.
Data uploaded by AStrickland and last updated on 2024-07-19 15:29:20.

Design Data

Rosetta Score for H315E: -1.189

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.