D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W120S

Expression Data

Yield (mg/mL): 0.74

Kinetic Assay Data

**slope of W120S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.40E-03	2.15E-03	2.25E-03
B	25.00	1.65E-03	1.80E-03	1.60E-03
C	8.33	8.00E-04	8.50E-04	9.50E-04
D	2.78	5.00E-04	5.50E-04	5.00E-04
E	0.93	4.50E-04	4.00E-04	5.00E-04
F	0.31	3.00E-04	3.00E-04	3.00E-04
G	0.10		3.00E-04	2.50E-04
H	0.03	0	0	0

Yield (mg/mL): 0.74 Dilution Factor: 10×

Protein purified on 24/7/2024 and assayed on 25/7/2025.
Data uploaded by wispwong and last updated on 2024-07-25 17:47:25.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.94E-01	1.79E-01	1.75E-01
B	25.00	1.39E-01	1.48E-01	1.43E-01
C	8.33	1.38E-01	1.21E-01	1.11E-01
D	2.78	6.85E-02	7.25E-02	8.18E-02
E	0.93	3.02E-02	3.41E-02	3.41E-02
F	0.31	1.12E-02	1.27E-02	1.20E-02
G	0.10	3.85E-03	3.95E-03	4.05E-03
H	0.03	1.55E-03	1.60E-03	1.90E-03

Yield (mg/mL): 3.25 Dilution Factor: 100×

Protein purified on 24/7/2024 and assayed on 25/7/2025.
Data uploaded by wispwong and last updated on 2024-07-25 17:45:52.

Thermostability Assay Data

Data missing or deleted from the database.

Reference Data for WT

**slope of W120S A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0.0006000	0.0006000
B	48.3		0.0008000	0.0007000
C	45.7	0.001100	0.0007000	0.0008000
D	42.4	0.05090	0.04300	0.05170
E	37.7	0.1758	0.1633	0.1704
F	33.6	0.2332		0.2267
G	31.3	0.2247	0.2171	0.2082
H	30.0	0.2180	0.2170	0.2001

Protein purified on 24/7/2024 and assayed on 25/7/2024.
Data uploaded by wispwong and last updated on 2024-07-25 20:38:27.

Design Data

Rosetta Score for W120S: 0.021

Citations

The unpublished data above were collected by students from The Hong Kong Polytechnic University. For inquiries, please contact Chi Ming: <chi-ming.cm.wong@polyu.edu.hk>.