Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 3.25

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.94E-01	1.79E-01	1.75E-01
	B	25.00	1.39E-01	1.48E-01	1.43E-01
	C	8.33	1.38E-01	1.21E-01	1.11E-01
	D	2.78	6.85E-02	7.25E-02	8.18E-02
	E	0.93	3.02E-02	3.41E-02	3.41E-02
	F	0.31	1.12E-02	1.27E-02	1.20E-02
	G	0.10	3.85E-03	3.95E-03	4.05E-03
	H	0.03	1.55E-03	1.60E-03	1.90E-03

Yield (mg/mL): 3.25     Dilution Factor:  100×

Download these data

Protein purified on 24/7/2024 and assayed on 25/7/2025.
Data uploaded by wispwong and last updated on 2024-07-25 17:45:52.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0.0006000	0.0006000
	B	48.3		0.0008000	0.0007000
	C	45.7	0.001100	0.0007000	0.0008000
	D	42.4	0.05090	0.04300	0.05170
	E	37.7	0.1758	0.1633	0.1704
	F	33.6	0.2332		0.2267
	G	31.3	0.2247	0.2171	0.2082
	H	30.0	0.2180	0.2170	0.2001

Download these data

Protein purified on 24/7/2024 and assayed on 25/7/2024.
Data uploaded by wispwong and last updated on 2024-07-25 20:38:27.

Citations

The unpublished data above were collected by students from The Hong Kong Polytechnic University. For inquiries, please contact Chi Ming: <chi-ming.cm.wong@polyu.edu.hk>.