Raw Data for BglB Variant H328K

Expression Data

SDS-PAGE
 2.2

Kinetic Assay Data

slope of H328K A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.54E-01 1.52E-01 1.54E-01
B 25.00 1.30E-01 1.29E-01 1.31E-01
C 8.33 1.21E-01 1.12E-01 1.12E-01
D 2.78 5.62E-02 5.62E-02 5.45E-02
E 0.93 3.49E-02 4.11E-02 3.74E-02
F 0.31 1.31E-02 1.34E-02 1.17E-02
G 0.10 7.50E-03 6.85E-03
H 0.03 2.95E-03 2.30E-03 1.45E-03
 2.2      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:27:35.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.88E-01 1.82E-01 1.79E-01
B 25.00 1.49E-01 1.52E-01 1.47E-01
C 8.33 1.35E-01 1.37E-01 1.39E-01
D 2.78 6.51E-02 6.22E-02 6.55E-02
E 0.93 4.34E-02 4.43E-02 4.18E-02
F 0.31 2.02E-02 1.64E-02 1.69E-02
G 0.10 9.45E-03 1.03E-02 7.65E-03
H 0.03 3.40E-03 3.45E-03 2.45E-03
 2.377      100×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 19:32:59.


Thermostability Assay Data

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 5.00E-04 4.00E-04
B 48.3 0 0
C 45.7 5.00E-04 6.00E-04 4.00E-04
D 42.4 8.50E-03 6.50E-03 5.20E-03
E 37.7 1.51E-01 1.48E-01
F 33.6 1.68E-01 1.66E-01 1.50E-01
G 31.3 1.67E-01 1.76E-01 1.69E-01
H 30.0 1.77E-01 1.82E-01 1.97E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:09:23.


Reference Data for WT

slope of H328K A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 7.00E-04
B 48.3 4.00E-04 5.00E-04 6.00E-04
C 45.7 6.00E-04 9.00E-04 7.00E-04
D 42.4 4.12E-02 3.96E-02 4.71E-02
E 37.7 2.14E-01 1.91E-01 2.10E-01
F 33.6 2.47E-01 2.35E-01 2.36E-01
G 31.3 2.18E-01 2.14E-01 2.05E-01
H 30.0 2.62E-01 2.56E-01 2.62E-01

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ghaziaabbas and last updated on 2024-07-25 20:10:03.


Citations

The unpublished data above were collected by students from Yale University. For inquiries, please contact Ghazia: <ghazia.abbas@yale.edu>.