D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A80P

Expression Data

Yield (mg/mL): 0.38

Kinetic Assay Data

**slope of A80P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.78E-02	2.98E-02	2.99E-02
B	25.00	2.28E-02	2.40E-02	2.39E-02
C	8.33	1.46E-02	1.57E-02	1.49E-02
D	2.78	8.85E-03	8.70E-03	9.90E-03
E	0.93	3.65E-03	4.60E-03	3.95E-03
F	0.31	1.35E-03	1.65E-03	1.65E-03
G	0.10	6.00E-04	6.00E-04	6.50E-04
H	0.03	3.50E-04	3.50E-04

Yield (mg/mL): 0.38 Dilution Factor: 10×

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:21:49.

Thermostability Assay Data

**slope of A80P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		0	0
B	48.3	0	9.00E-04	4.00E-04
C	45.7	0		0
D	42.4	6.00E-04	7.00E-04	6.00E-04
E	37.7	2.90E-03	2.50E-03	2.60E-03
F	33.6	3.20E-03	3.70E-03	3.80E-03
G	31.3	7.00E-03	5.00E-03	5.60E-03
H	30.0	8.20E-03	7.20E-03	7.40E-03

Protein purified on 7/24/24 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:27:14.

Reference Data for WT

**slope of A80P A_{420 nm} (min⁻¹)**
	T (°C)	1	2	3
A	50.0		6.00E-04	6.00E-04
B	48.3		8.00E-04	7.00E-04
C	45.7	1.10E-03	7.00E-04	8.00E-04
D	42.4	5.09E-02	4.30E-02	5.17E-02
E	37.7	1.76E-01	1.63E-01	1.70E-01
F	33.6	2.33E-01		2.27E-01
G	31.3	2.25E-01	2.17E-01	2.08E-01
H	30.0	2.18E-01	2.17E-01	2.00E-01

Protein purified on 7/24/25 and assayed on 7/25/24.
Data uploaded by ssilver and last updated on 2024-07-25 19:50:41.

Citations

The unpublished data above were collected by students from Elmhurst University. For inquiries, please contact Sunshine Silver: <sunshine.silver@elmhurst.edu>.