Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.139611765	0.139630294
	B	25.00	0.128248971	0.119688676
	C	8.33	0.086550882	0.086962794
	D	2.78	0.039723529	0.043826029
	E	0.93	0.022215	0.021438676
	F	0.31	0.011845882	0.011395441
	G	0.10	0.004566618	0.003670147
	H	0.03	0.002452941	0.001793088

Yield (mg/mL): 0.83     Dilution Factor:  100×

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Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	0.664885714
	B	31.8	0.79277
	C	33.6	0.77854
	D	35.5	0.639442857
	E	37.3	0.7241
	F	39.1	0.67721
	G	40.9	0.258779341
	H	42.7	0.03094044
	I	44.5	0.00119706
	J	46.4	0.00043618
	K	48.1	0.00025809
	L	50.0	0.00022632

Download these data

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:16.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.