Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 2.377

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	1.88E-01	1.82E-01	1.79E-01
	B	25.00	1.49E-01	1.52E-01	1.47E-01
	C	8.33	1.35E-01	1.37E-01	1.39E-01
	D	2.78	6.51E-02	6.22E-02	6.55E-02
	E	0.93	4.34E-02	4.43E-02	4.18E-02
	F	0.31	2.02E-02	1.64E-02	1.69E-02
	G	0.10	9.45E-03	1.03E-02	7.65E-03
	H	0.03	3.40E-03	3.45E-03	2.45E-03

Yield (mg/mL): 2.377     Dilution Factor:  100×

Download these data

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 19:56:47.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0		0	7.00E-04
	B	48.3	4.00E-04	5.00E-04	6.00E-04
	C	45.7	6.00E-04	9.00E-04	7.00E-04
	D	42.4	4.12E-02	3.96E-02	4.71E-02
	E	37.7	2.14E-01	1.91E-01	2.10E-01
	F	33.6	2.47E-01	2.35E-01	2.36E-01
	G	31.3	2.18E-01	2.14E-01	2.05E-01
	H	30.0	2.62E-01	2.56E-01	2.62E-01

Download these data

Protein purified on 7/24/2024 and assayed on 7/25/2024.
Data uploaded by montezano and last updated on 2024-07-25 20:00:11.

Citations

The unpublished data above were collected by students from . For inquiries, please contact Daniel Montezano: <montezano@ku.edu>.