Raw Data for BglB Variant N220S

Expression Data

Yield (mg/mL): 0.533

Kinetic Assay Data

slope of N220S A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.0708	0.0593	0.0766
	B	25.00	0.0258	0.0277	0.0358
	C	8.33	0.0088	0.0104	0.0106
	D	2.78	0.0031	0.0031	0.003
	E	0.93	0.0007	0.0006	0.0009
	F	0.31	0.0008	0.0005	0.0006
	G	0.10		0.0004	0.0004
	H	0.03		0.00003	0.00006

Yield (mg/mL): 0.533     Dilution Factor:  100×

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Protein purified on 4/14/2021 and assayed on 4/24/2021.
Data uploaded by saraheliadams and last updated on 2022-07-28 15:14:57.

Reference Data for WT

Wild-type data not found. It may have been rejected in the curation process and deleted from the database.

Thermostability Assay Data

slope of N220S A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	6.11E-02	6.29E-02
	B	31.8	4.65E-02	6.66E-02
	C	33.6	5.53E-02	5.60E-02
	D	35.5	0.0523	0.052
	E	37.3	0.0464	0.044
	F	39.1	0.0401	0.0384
	G	40.9	0.0223	0.0218
	H	42.7	0.0009	0.0012
	I	44.5	0.0002	0
	J	46.4	0	0
	K	48.1	0.0001	0
	L	50.0	0	0

Download these data

Protein purified on 4/16/2021 and assayed on 4/24/2021.
Data uploaded by saraheliadams and last updated on 2022-07-28 15:10:26.

Reference Data for WT

slope of N220S A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	0.664885714
	B	31.8	0.79277
	C	33.6	0.77854
	D	35.5	0.639442857
	E	37.3	0.7241
	F	39.1	0.67721
	G	40.9	0.258779341
	H	42.7	0.03094044
	I	44.5	0.00119706
	J	46.4	0.00043618
	K	48.1	0.00025809
	L	50.0	0.00022632

Download these data

Protein purified on 4/16/21 and assayed on 4/24/21.
Data uploaded by Gyasu and last updated on 2022-07-28 11:26:16.

Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.