D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C167N

Expression Data

Yield (mg/mL): 1.72

Kinetic Assay Data

**slope of C167N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		0.09355	0.09240
B	25.00	0.1114	0.08720	0.1328
C	8.33	0.09310	0.1540	0.1248
D	2.78	0.08625	0.05590	0.06870
E	0.93		0.03380	0.03070
F	0.31	0.01815	0.01360	0.008500
G	0.10	0.004800	0.005100	0.004500
H	0.03	0.0002500	0.0002500

Yield (mg/mL): 1.72 Dilution Factor: 100×

Protein purified on 7/9/2024 and assayed on 7/10/2024.
Data uploaded by alxlo and last updated on 2024-07-26 14:54:16.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.10E-01	1.95E-01	2.08E-01
B	25.00	1.93E-01	1.90E-01	1.83E-01
C	8.33	1.38E-01	1.49E-01	1.50E-01
D	2.78	9.79E-02	9.52E-02	9.61E-02
E	0.93	6.14E-02	5.14E-02	5.32E-02
F	0.31	2.23E-02	2.01E-02	2.81E-02
G	0.10	9.65E-03	7.65E-03	1.18E-02
H	0.03	3.00E-04	3.00E-04	3.00E-04

Yield (mg/mL): 2.682 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-26 15:07:25.

Design Data

Rosetta Score for C167N: 4.143

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.