D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant R427L

Expression Data

Yield (mg/mL): 2.2

Kinetic Assay Data

**slope of R427L A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.42E-01	2.43E-01	2.43E-01
B	25.00	1.90E-01	1.94E-01	1.96E-01
C	8.33	1.58E-01	1.52E-01	1.57E-01
D	2.78	1.02E-01	9.68E-02	1.02E-01
E	0.93	5.22E-02	4.70E-02	5.15E-02
F	0.31	2.19E-02	1.89E-02	2.14E-02
G	0.10	8.35E-03	6.82E-03	7.53E-03
H	0.03		1.20E-04	1.30E-04

Yield (mg/mL): 2.2 Dilution Factor: 100×

Protein purified on 7/23/2024 and assayed on 7/29/2024.
Data uploaded by IzzyG and last updated on 2024-07-29 21:46:27.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.43E-01	2.13E-01	2.18E-01
B	25.00	1.83E-01	1.71E-01	1.78E-01
C	8.33	1.42E-01	1.30E-01	1.38E-01
D	2.78	8.47E-02	8.54E-02	8.81E-02
E	0.93	3.66E-02	3.88E-02	4.21E-02
F	0.31	1.51E-02	1.54E-02	1.78E-02
G	0.10	5.30E-03	5.50E-03	6.05E-03
H	0.03	2.50E-04	2.00E-04	3.00E-04

Yield (mg/mL): 2.7 Dilution Factor: 100×

Protein purified on 7/23/2024 and assayed on 7/29/2024.
Data uploaded by IzzyG and last updated on 2024-07-29 20:39:34.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.