D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W407D

Expression Data

Yield (mg/mL): 0.758

Kinetic Assay Data

**slope of W407D A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0	3.00E-04	2.00E-04
B	25.00	0	0	0
C	8.33		0	0
D	2.78	0	0
E	0.93		2.00E-04	0
F	0.31		0	2.50E-04
G	0.10		2.50E-04	1.50E-04
H	0.03			2.00E-04

Yield (mg/mL): 0.758 Dilution Factor: 10×

Protein purified on 7/9/2024 and assayed on 7/10/2024.
Data uploaded by brianhle and last updated on 2024-07-30 16:05:17.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.21E-01	2.90E-01
B	25.00	3.33E-01	2.44E-01	2.82E-01
C	8.33	1.92E-01	1.88E-01	1.76E-01
D	2.78	9.26E-02	1.19E-01	1.32E-01
E	0.93	5.14E-02	6.22E-02	5.79E-02
F	0.31	2.21E-02	2.21E-02	1.86E-02
G	0.10	6.75E-03	7.65E-03	6.60E-03
H	0.03	0		0

Yield (mg/mL): 3.048 Dilution Factor: 100×

Protein purified on 7/9/2024 and assayed on 7/10/2024.
Data uploaded by brianhle and last updated on 2024-12-19 19:31:46.

Design Data

Rosetta Score for W407D: -3.248

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.