D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E340A

Expression Data

Yield (mg/mL): 3.416

Kinetic Assay Data

**slope of E340A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		2.72E-01	2.48E-01
B	25.00		2.52E-01	2.56E-01
C	8.33		1.93E-01	1.94E-01
D	2.78	1.09E-01	1.10E-01	1.38E-01
E	0.93	8.21E-02		7.36E-02
F	0.31	5.05E-02	5.24E-02
G	0.10	3.24E-02	1.95E-02	7.55E-03
H	0.03	3.00E-04	0	2.00E-04

Yield (mg/mL): 3.416 Dilution Factor: 100×

Protein purified on 7/29/24 and assayed on 7/30/24.
Data uploaded by Azweiback and last updated on 2024-07-31 14:33:41.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.10E-01	1.95E-01	2.08E-01
B	25.00	1.93E-01	1.90E-01	1.83E-01
C	8.33	1.38E-01	1.49E-01	1.50E-01
D	2.78	9.79E-02	9.52E-02	9.61E-02
E	0.93	6.14E-02	5.14E-02	5.32E-02
F	0.31	2.23E-02	2.01E-02	2.81E-02
G	0.10	9.65E-03	7.65E-03	1.18E-02
H	0.03	3.00E-04	3.00E-04	3.00E-04

Yield (mg/mL): 2.682 Dilution Factor: 100×

Protein purified on 7/9/24 and assayed on 7/10/24.
Data uploaded by Azweiback and last updated on 2024-07-26 15:07:25.

Design Data

Rosetta Score for E340A: 3.972

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.