Raw Data for BglB Variant R427W

Expression Data

SDS-PAGE
 0.312

Kinetic Assay Data

slope of R427W A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.026990441 0.032032647 0.022799706
B 25.00 0.033469412 0.031857941 0.035239853
C 8.33 0.042219412 0.022039853 0.034058676
D 2.78 0.027655 0.0255925 0.023717794
E 0.93 0.017137059 0.015658971 0.016891324
F 0.31 0.004584853 0.004256029 0.004161029
G 0.10 0.001510882 0.001483824
H 0.03 0.001257206 0.001152647
 0.312      10×

Protein purified on 4/21/2021 and assayed on 4/21/2021.
Data uploaded by Oliverhp and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 1.12E-01 1.19E-01
B 25.00 9.11E-02 9.53E-02
C 8.33 8.12E-02 8.28E-02
D 2.78 5.24E-02 3.59E-02
E 0.93 2.76E-02 2.27E-02
F 0.31 1.25E-02 9.84E-03
G 0.10 3.03E-03 3.03E-03
H 0.03 5.47E-04 1.28E-03
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of R427W A420 nm (min−1)
T (°C) 1 2
A 30.0 0.27553022 0.333530909
B 31.8 0.308224825 0.296127972
C 33.6 0.315138182 0.308611818
D 35.5 0.309118182 0.296111888
E 37.3 0.314035455 0.292918531
F 39.1 0.288451748 0.269898352
G 40.9 0.098850714 0.093853214
H 42.7 0.002711071 0.002296429
I 44.5 0.000242143 0.000306786
J 46.6 0.000319286 1.89286E-05
K 48.1 0.000205714 2.89286E-05
L 50.0 0.00027 8.39286E-05

Protein purified on 4/21/2021 and assayed on 4/28/2021.
Data uploaded by Oliverhp and last updated on 2022-07-28 15:05:55.


Reference Data for WT

slope of R427W A420 nm (min−1)
T (°C) 1 2
A 30.0 4.648E-01 4.283E-01
B 31.8 4.733E-01 4.543E-01
C 33.6 4.575E-01 4.692E-01
D 35.5 4.611E-01 4.592E-01
E 37.3 4.559E-01 4.536E-01
F 39.1 3.877E-01 4.000E-01
G 40.9 1.069E-01 1.150E-01
H 42.7 4.025E-03 3.058E-03
I 44.5 1.635E-04 4.815E-04
J 46.6 0 2.675E-04
K 48.1 2.794E-04 6.750E-05
L 50.0 3.738E-04 1.060E-04

Protein purified on 4/20/21 and assayed on 4/28/21.
Data uploaded by lmelink and last updated on 2022-07-28 13:43:07.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.