D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220F

Expression Data

Yield (mg/mL): 1.004

Kinetic Assay Data

**slope of N220F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.43E-02	2.55E-02	2.48E-02
B	25.00	2.60E-02	2.49E-02	2.33E-02
C	8.33	2.60E-02	2.39E-02	2.30E-02
D	2.78			2.33E-02
E	0.93	2.18E-02	2.03E-02	2.10E-02
F	0.31	1.25E-02	1.38E-02	1.40E-02
G	0.10	6.90E-03	7.20E-03	7.05E-03
H	0.03	0	1.20E-03	6.00E-04

Yield (mg/mL): 1.004 Dilution Factor: 100×

Protein purified on 7/31/2024 and assayed on 7/31/2024.
Data uploaded by mcorr128 and last updated on 2024-08-01 15:01:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.61E-01	4.48E-01	4.76E-01
B	25.00	3.57E-01	3.59E-01	3.85E-01
C	8.33	2.74E-01	2.63E-01	2.94E-01
D	2.78	1.60E-01	1.74E-01	1.74E-01
E	0.93	7.23E-02	7.49E-02	7.34E-02
F	0.31	2.67E-02	2.85E-02	2.87E-02
G	0.10	9.60E-03	9.75E-03	9.15E-03
H	0.03	0	0	0

Yield (mg/mL): 6.655 Dilution Factor: 100×

Protein purified on 3/22/2024 and assayed on 3/29/2024.
Data uploaded by Dominic and last updated on 2024-04-19 13:41:56.

Citations

The unpublished data above were collected by students from Florida International University. For inquiries, please contact Jaime Mayoral: <mayoralj@fiu.edu>.