D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant E177A

Expression Data

Yield (mg/mL): 1.746

Kinetic Assay Data

**slope of E177A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.25E-01	1.25E-01	1.25E-01
B	25.00	9.21E-02	8.91E-02	9.21E-02
C	8.33	6.30E-02	6.09E-02	6.26E-02
D	2.78	3.62E-02	3.57E-02	3.61E-02
E	0.93	1.61E-02	1.59E-02	1.63E-02
F	0.31	5.95E-03	5.95E-03	6.05E-03
G	0.10	2.20E-03	2.30E-03	2.20E-03
H	0.03	8.50E-04	8.00E-04	8.50E-04

Yield (mg/mL): 1.746 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:23:18.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.62E-01	1.67E-01	1.65E-01
B	25.00	1.13E-01	1.28E-01	1.34E-01
C	8.33	9.03E-02	9.13E-02	9.97E-02
D	2.78	5.71E-02	5.91E-02	5.88E-02
E	0.93	2.88E-02	2.99E-02	2.78E-02
F	0.31	1.06E-02	1.03E-02	1.13E-02
G	0.10	3.80E-03	3.85E-03	4.00E-03
H	0.03	1.65E-03	1.50E-03	1.55E-03

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:20:52.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.