D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A306S

Expression Data

Yield (mg/mL): 1.852

Kinetic Assay Data

**slope of A306S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.00E-01	2.03E-01	1.98E-01
B	25.00	1.49E-01	1.50E-01	1.54E-01
C	8.33	1.09E-01	1.11E-01	1.14E-01
D	2.78	6.58E-02	6.54E-02	6.48E-02
E	0.93	3.00E-02	2.95E-02	2.95E-02
F	0.31	1.15E-02	1.16E-02	1.15E-02
G	0.10	4.05E-03	4.05E-03	3.90E-03
H	0.03	1.75E-03	1.50E-03	1.55E-03

Yield (mg/mL): 1.852 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:34:05.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.65E-01	1.65E-01	1.67E-01
B	25.00	1.28E-01	1.26E-01	1.26E-01
C	8.33	9.38E-02	9.22E-02	9.49E-02
D	2.78	5.63E-02	5.44E-02	5.52E-02
E	0.93	2.54E-02	2.48E-02	2.53E-02
F	0.31	9.90E-03	9.95E-03	9.60E-03
G	0.10	3.75E-03	3.50E-03	3.50E-03
H	0.03	1.35E-03		1.40E-03

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:37:27.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.