D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A356T

Expression Data

Yield (mg/mL): 1.902

Kinetic Assay Data

**slope of A356T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.62E-01	1.66E-01	1.61E-01
B	25.00	1.38E-01	1.37E-01	1.34E-01
C	8.33	1.15E-01	1.17E-01	1.18E-01
D	2.78	8.11E-02	7.82E-02	8.18E-02
E	0.93	4.21E-02	3.91E-02	4.28E-02
F	0.31	1.78E-02	1.63E-02	1.82E-02
G	0.10	6.35E-03	5.90E-03	6.60E-03
H	0.03	2.45E-03	2.00E-03	2.40E-03

Yield (mg/mL): 1.902 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:41:44.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.65E-01	1.65E-01	1.67E-01
B	25.00	1.28E-01	1.26E-01	1.26E-01
C	8.33	9.38E-02	9.22E-02	9.49E-02
D	2.78	5.63E-02	5.44E-02	5.52E-02
E	0.93	2.54E-02	2.48E-02	2.53E-02
F	0.31	9.90E-03	9.95E-03	9.60E-03
G	0.10	3.75E-03	3.50E-03	3.50E-03
H	0.03	1.35E-03		1.40E-03

Yield (mg/mL): 1.556 Dilution Factor: 100×

Protein purified on 7/17/23 and assayed on 7/18/24.
Data uploaded by mvlouie and last updated on 2024-08-05 11:37:27.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.