D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T13S

Expression Data

Yield (mg/mL): 0.939

Kinetic Assay Data

**slope of T13S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		5.76E-02	5.65E-02
C	8.33		5.11E-02	5.09E-02
D	2.78		3.18E-02	3.21E-02
E	0.93		1.46E-02	1.47E-02
F	0.31		5.65E-03	5.70E-03
G	0.10		2.30E-03	2.15E-03
H	0.03	9.00E-04	9.00E-04	9.50E-04

Yield (mg/mL): 0.939 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 15:53:22.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.39E-01	1.32E-01
B	25.00	1.24E-01	1.16E-01	1.11E-01
C	8.33	9.65E-02	8.68E-02	8.47E-02
D	2.78	5.46E-02	5.19E-02	5.04E-02
E	0.93	2.35E-02	2.30E-02	2.32E-02
F	0.31	8.60E-03	8.85E-03	8.80E-03
G	0.10	3.05E-03	3.05E-03	3.10E-03
H	0.03	1.25E-03	1.15E-03	1.20E-03

Yield (mg/mL): 1.719 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 15:46:25.

Design Data

Rosetta Score for T13S: 2.802

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.