D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D233I

Expression Data

Yield (mg/mL): 0.579

Kinetic Assay Data

**slope of D233I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00
B	25.00		5.05E-03	4.95E-03
C	8.33	4.00E-03	3.95E-03	3.95E-03
D	2.78	2.25E-03	2.40E-03	2.25E-03
E	0.93	1.10E-03	1.00E-03	1.15E-03
F	0.31	7.00E-04	5.50E-04	6.00E-04
G	0.10	3.00E-04	3.50E-04	3.50E-04
H	0.03	3.00E-04	3.50E-04	4.00E-04

Yield (mg/mL): 0.579 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:11:02.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.39E-01	1.32E-01
B	25.00	1.24E-01	1.16E-01	1.11E-01
C	8.33	9.65E-02	8.68E-02	8.47E-02
D	2.78	5.46E-02	5.19E-02	5.04E-02
E	0.93	2.35E-02	2.30E-02	2.32E-02
F	0.31	8.60E-03	8.85E-03	8.80E-03
G	0.10	3.05E-03	3.05E-03	3.10E-03
H	0.03	1.25E-03	1.15E-03	1.20E-03

Yield (mg/mL): 1.719 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 15:46:25.

Design Data

Rosetta Score for D233I: 16.228

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.