D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant S302A

Expression Data

Yield (mg/mL): 2.095

Kinetic Assay Data

**slope of S302A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.91E-01	1.79E-01
B	25.00		1.56E-01	1.50E-01
C	8.33		1.21E-01	1.17E-01
D	2.78		7.15E-02	7.06E-02
E	0.93	3.59E-02	3.38E-02	3.16E-02
F	0.31	1.33E-02	1.29E-02	1.22E-02
G	0.10	4.80E-03	4.65E-03	4.15E-03
H	0.03	1.75E-03	1.70E-03	1.60E-03

Yield (mg/mL): 2.095 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:26:24.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.89E-01	1.82E-01
B	25.00		1.55E-01	1.55E-01
C	8.33	1.37E-01	1.26E-01	1.14E-01
D	2.78	8.19E-02	8.33E-02	6.98E-02
E	0.93	3.60E-02	4.17E-02	3.05E-02
F	0.31	1.30E-02		1.17E-02
G	0.10	4.60E-03		4.25E-03
H	0.03	1.75E-03	2.35E-03	1.55E-03

Yield (mg/mL): 2.299 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:19:50.

Design Data

Rosetta Score for S302A: -0.754

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.