D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A408H

Expression Data

Yield (mg/mL): 1.501

Kinetic Assay Data

**slope of A408H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.02E-01	9.44E-02	9.19E-02
B	25.00	7.30E-02	6.55E-02	6.71E-02
C	8.33	4.62E-02	4.23E-02	4.12E-02
D	2.78	2.36E-02	2.37E-02	2.10E-02
E	0.93	9.35E-03	1.04E-02	8.35E-03
F	0.31	3.45E-03	4.15E-03	3.20E-03
G	0.10	1.35E-03	1.50E-03	1.15E-03
H	0.03	6.00E-04	6.00E-04	5.00E-04

Yield (mg/mL): 1.501 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:39:17.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.89E-01	1.82E-01
B	25.00		1.55E-01	1.55E-01
C	8.33	1.37E-01	1.26E-01	1.14E-01
D	2.78	8.19E-02	8.33E-02	6.98E-02
E	0.93	3.60E-02	4.17E-02	3.05E-02
F	0.31	1.30E-02		1.17E-02
G	0.10	4.60E-03		4.25E-03
H	0.03	1.75E-03	2.35E-03	1.55E-03

Yield (mg/mL): 2.299 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:19:50.

Design Data

Rosetta Score for A408H: -0.049

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.