D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L171I

Expression Data

Yield (mg/mL): 1.525

Kinetic Assay Data

**slope of L171I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.40E-01	1.43E-01	1.35E-01
B	25.00		1.13E-01	1.09E-01
C	8.33		7.50E-02	7.23E-02
D	2.78	4.04E-02	4.45E-02	4.30E-02
E	0.93	1.80E-02	1.95E-02	1.98E-02
F	0.31	7.50E-03	7.80E-03	7.45E-03
G	0.10	2.70E-03	2.95E-03	2.75E-03
H	0.03	1.05E-03	9.50E-04	1.05E-03

Yield (mg/mL): 1.525 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:51:31.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.27E-01	1.29E-01
B	25.00		1.07E-01	1.06E-01
C	8.33	8.57E-02	7.72E-02	8.17E-02
D	2.78	5.20E-02	4.64E-02	4.79E-02
E	0.93	2.04E-02	2.07E-02	2.18E-02
F	0.31	8.15E-03	8.10E-03	8.60E-03
G	0.10	3.30E-03	2.95E-03	3.10E-03
H	0.03	1.20E-03	1.15E-03	1.25E-03

Yield (mg/mL): 1.575 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:46:19.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.