D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A306N

Expression Data

Yield (mg/mL): 1.769

Kinetic Assay Data

**slope of A306N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.86E-01	1.92E-01	1.92E-01
B	25.00	1.69E-01	1.60E-01	1.63E-01
C	8.33	1.22E-01	1.19E-01	1.19E-01
D	2.78	7.60E-02	7.18E-02	7.31E-02
E	0.93	3.54E-02	3.34E-02	3.31E-02
F	0.31	1.35E-02	1.30E-02	1.27E-02
G	0.10	4.80E-03	4.60E-03	4.55E-03
H	0.03	1.80E-03	1.65E-03	1.70E-03

Yield (mg/mL): 1.769 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 17:02:32.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.73E-01	1.65E-01	1.61E-01
B	25.00	1.44E-01	1.37E-01	1.34E-01
C	8.33	1.08E-01	1.02E-01	1.01E-01
D	2.78	7.04E-02	6.09E-02	6.13E-02
E	0.93	3.23E-02	2.91E-02	2.80E-02
F	0.31	1.26E-02	1.14E-02	1.07E-02
G	0.10	4.20E-03	3.90E-03	3.85E-03
H	0.03	1.65E-03	1.45E-03	1.40E-03

Yield (mg/mL): 1.727 Dilution Factor: 100×

Protein purified on 8/14/2024 and assayed on 8/15/2024.
Data uploaded by AStrickland and last updated on 2024-08-19 16:58:44.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.