D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T116I

Expression Data

Yield (mg/mL): 0.897

Kinetic Assay Data

**slope of T116I A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	3.14E-02	3.12E-02	3.52E-02
B	25.00	2.40E-02	2.13E-02	2.12E-02
C	8.33	1.34E-02	1.36E-02	1.30E-02
D	2.78	8.55E-03	8.00E-03	8.15E-03
E	0.93	4.45E-03	4.60E-03	4.50E-03
F	0.31	1.80E-03	2.00E-03	1.80E-03
G	0.10	8.00E-04	8.50E-04	6.50E-04
H	0.03	3.00E-04	6.00E-04	4.50E-04

Yield (mg/mL): 0.897 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 13:47:09.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.46E-01	1.18E-01	1.39E-01
B	25.00	1.20E-01	1.20E-01	1.17E-01
C	8.33	8.68E-02	8.57E-02	8.93E-02
D	2.78	4.88E-02	5.00E-02	5.17E-02
E	0.93	2.29E-02	2.32E-02	2.27E-02
F	0.31	9.15E-03	8.70E-03	8.90E-03
G	0.10	3.30E-03	3.05E-03	3.15E-03
H	0.03	2.00E-03	3.00E-03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:15:02.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.