D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant L219M

Expression Data

Yield (mg/mL): 0.821

Kinetic Assay Data

**slope of L219M A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	5.65E-02	5.21E-02	6.74E-02
B	25.00	4.40E-02	4.86E-02	4.75E-02
C	8.33	3.52E-02	3.52E-02	3.56E-02
D	2.78	2.12E-02	2.12E-02	2.07E-02
E	0.93	1.07E-02	1.03E-02	1.04E-02
F	0.31	4.20E-03	3.80E-03	4.05E-03
G	0.10	1.60E-03	1.50E-03	1.55E-03
H	0.03	7.00E-04	6.50E-04	7.00E-04

Yield (mg/mL): 0.821 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:27:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.46E-01	1.18E-01	1.39E-01
B	25.00	1.20E-01	1.20E-01	1.17E-01
C	8.33	8.68E-02	8.57E-02	8.93E-02
D	2.78	4.88E-02	5.00E-02	5.17E-02
E	0.93	2.29E-02	2.32E-02	2.27E-02
F	0.31	9.15E-03	8.70E-03	8.90E-03
G	0.10	3.30E-03	3.05E-03	3.15E-03
H	0.03	2.00E-03	3.00E-03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:15:02.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.