D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant F253Y

Expression Data

Yield (mg/mL): 1.149

Kinetic Assay Data

**slope of F253Y A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.85E-02	9.19E-02	8.95E-02
B	25.00	6.94E-02	6.84E-02	6.75E-02
C	8.33	4.97E-02	4.99E-02	4.80E-02
D	2.78	2.89E-02	2.84E-02	2.67E-02
E	0.93	1.25E-02	1.26E-02	1.28E-02
F	0.31	4.55E-03	4.70E-03	4.55E-03
G	0.10	1.60E-03	1.85E-03	1.70E-03
H	0.03	8.00E-04	1.00E-03	7.50E-04

Yield (mg/mL): 1.149 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:29:00.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.41E-01	1.48E-01	1.43E-01
B	25.00	1.10E-01	1.11E-01	1.12E-01
C	8.33	7.98E-02	8.27E-02	8.28E-02
D	2.78	4.69E-02	4.76E-02	4.85E-02
E	0.93	2.08E-02	2.11E-02	2.03E-02
F	0.31		7.55E-03	7.85E-03
G	0.10	3.05E-03	2.80E-03	2.70E-03
H	0.03	1.25E-03	1.40E-03	1.25E-03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:22:46.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.