D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H328A

Expression Data

Yield (mg/mL): 0.769

Kinetic Assay Data

**slope of H328A A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.79E-02	8.80E-02	8.96E-02
B	25.00	7.33E-02	6.85E-02	6.96E-02
C	8.33	4.91E-02	4.74E-02	4.75E-02
D	2.78	2.59E-02	2.59E-02	2.59E-02
E	0.93	1.09E-02	1.05E-02	1.06E-02
F	0.31	4.05E-03	4.00E-03	3.75E-03
G	0.10	1.50E-03	1.60E-03	1.40E-03
H	0.03	9.00E-04	1.05E-03	6.50E-04

Yield (mg/mL): 0.769 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:35:35.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.41E-01	1.48E-01	1.43E-01
B	25.00	1.10E-01	1.11E-01	1.12E-01
C	8.33	7.98E-02	8.27E-02	8.28E-02
D	2.78	4.69E-02	4.76E-02	4.85E-02
E	0.93	2.08E-02	2.11E-02	2.03E-02
F	0.31		7.55E-03	7.85E-03
G	0.10	3.05E-03	2.80E-03	2.70E-03
H	0.03	1.25E-03	1.40E-03	1.25E-03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:22:46.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.