D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N354S

Expression Data

Yield (mg/mL): 1.009

Kinetic Assay Data

**slope of N354S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.21E-02	1.30E-02	1.24E-02
B	25.00	9.90E-03	1.01E-02	1.00E-02
C	8.33	7.45E-03	7.35E-03	7.85E-03
D	2.78	5.05E-03	5.10E-03	5.15E-03
E	0.93	2.40E-03	2.65E-03	2.55E-03
F	0.31	1.20E-03	1.25E-03	1.30E-03
G	0.10	5.50E-04	5.50E-04
H	0.03	8.50E-04	0	1.25E-03

Yield (mg/mL): 1.009 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:40:15.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.42E-01	1.44E-01	1.37E-01
B	25.00	1.07E-01	1.14E-01	1.12E-01
C	8.33	8.15E-02	8.26E-02	7.96E-02
D	2.78	4.69E-02	4.71E-02	4.17E-02
E	0.93	2.13E-02	2.09E-02	1.94E-02
F	0.31	8.05E-03	7.95E-03	7.35E-03
G	0.10	3.05E-03	3.10E-03	3.00E-03
H	0.03	1.15E-03	1.15E-03	1.55E-03

Yield (mg/mL): 1.401 Dilution Factor: 100×

Protein purified on 8/23/24 and assayed on 8/29/24.
Data uploaded by mvlouie and last updated on 2024-09-04 14:41:36.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Michelle Louie: <mvlouie@ucdavis.edu>.