D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant C167N

Expression Data

Yield (mg/mL): 2.651

Kinetic Assay Data

**slope of C167N A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.51E-01	2.55E-01	2.62E-01
B	25.00	1.93E-01	2.03E-01	2.03E-01
C	8.33	1.24E-01	1.53E-01	1.47E-01
D	2.78	6.88E-02	7.47E-02	8.14E-02
E	0.93	3.23E-02	3.37E-02	3.66E-02
F	0.31	1.09E-02	1.02E-02	1.30E-02
G	0.10	4.55E-03	4.30E-03	4.80E-03
H	0.03	0	0

Yield (mg/mL): 2.651 Dilution Factor: 100×

Protein purified on 8/23/2024 and assayed on 8/27/2024.
Data uploaded by jedikayle and last updated on 2024-09-05 13:16:11.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	7.97E-01	7.03E-01	7.18E-01
B	25.00	7.11E-01	7.42E-01
C	8.33	8.11E-01	6.47E-01
D	2.78	7.96E-01	7.97E-01	7.45E-01
E	0.93	3.91E-01	3.93E-01	3.55E-01
F	0.31	1.63E-01	1.40E-01	1.42E-01
G	0.10	4.95E-02	4.70E-02	4.03E-02
H	0.03		0	0

Yield (mg/mL): 2.73 Dilution Factor: 10×

Protein purified on 8/23/2024 and assayed on 8/27/2024.
Data uploaded by jedikayle and last updated on 2024-09-05 13:11:45.

Design Data

Rosetta Score for C167N: -17.236

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.