D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant N220F

Expression Data

Yield (mg/mL): 2.302

Kinetic Assay Data

**slope of N220F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	9.41E-02	1.02E-01	1.03E-01
B	25.00	8.83E-02	9.12E-02	9.45E-02
C	8.33	8.26E-02	8.94E-02
D	2.78
E	0.93	7.30E-02	7.86E-02	7.26E-02
F	0.31	3.61E-02	4.04E-02	4.26E-02
G	0.10	1.54E-02	1.75E-02	1.97E-02
H	0.03		3.50E-04	3.50E-04

Yield (mg/mL): 2.302 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/4/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:31:49.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.21E-01	1.06E-01
B	25.00	9.95E-02	8.89E-02	9.32E-02
C	8.33	7.30E-02	6.83E-02	7.14E-02
D	2.78	4.39E-02	4.79E-02	4.88E-02
E	0.93	1.93E-02	2.16E-02	2.27E-02
F	0.31	7.10E-03	7.20E-03	7.90E-03
G	0.10	2.60E-03	2.95E-03	3.15E-03
H	0.03		3.00E-04

Yield (mg/mL): 2.3 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/5/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:42:55.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.