D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant W264H

Expression Data

Yield (mg/mL): 1.94

Kinetic Assay Data

**slope of W264H A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.36E-01	1.51E-01	1.44E-01
B	25.00	1.19E-01	1.07E-01	1.24E-01
C	8.33	9.35E-02	1.09E-01	1.09E-01
D	2.78	5.73E-02	6.30E-02	6.15E-02
E	0.93	3.16E-02	2.88E-02	2.74E-02
F	0.31	1.05E-02	1.03E-02	1.03E-02
G	0.10	3.70E-03	3.85E-03	3.50E-03
H	0.03	3.00E-04	2.50E-04

Yield (mg/mL): 1.94 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/5/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:57:01.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00		1.21E-01	1.06E-01
B	25.00	9.95E-02	8.89E-02	9.32E-02
C	8.33	7.30E-02	6.83E-02	7.14E-02
D	2.78	4.39E-02	4.79E-02	4.88E-02
E	0.93	1.93E-02	2.16E-02	2.27E-02
F	0.31	7.10E-03	7.20E-03	7.90E-03
G	0.10	2.60E-03	2.95E-03	3.15E-03
H	0.03		3.00E-04

Yield (mg/mL): 2.3 Dilution Factor: 100×

Protein purified on 8/28/2024 and assayed on 9/5/2024.
Data uploaded by jishy7 and last updated on 2024-09-18 13:42:55.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.