D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant M114S

Expression Data

Yield (mg/mL): 0.682

Kinetic Assay Data

**slope of M114S A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	1.00E-02	9.50E-03	9.00E-03
B	25.00	8.00E-03	7.50E-03	7.00E-03
C	8.33	6.00E-03	6.00E-03	6.00E-03
D	2.78	3.00E-03	3.50E-03	3.00E-03
E	0.93	1.50E-03	1.50E-03	1.50E-03
F	0.31		5.00E-04	5.00E-04
G	0.10	5.00E-04	5.00E-04	5.00E-04
H	0.03	0.00E+00	1.48E-16

Yield (mg/mL): 0.682 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 21:51:10.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.00E-01	7.42E-01	8.28E-01
B	25.00	6.01E-01	6.25E-01	6.55E-01
C	8.33	5.00E-01	4.69E-01	5.05E-01
D	2.78	3.21E-01	3.51E-01	3.74E-01
E	0.93	1.48E-01	1.40E-01	1.62E-01
F	0.31	5.70E-02	5.80E-02	6.75E-02
G	0.10	2.10E-02	2.05E-02	2.20E-02
H	0.03	1.00E-03		1.00E-03

Yield (mg/mL): 7.569 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 19:16:59.

Design Data

Rosetta Score for M114S: 5.021

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.