D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant A88E

Expression Data

Yield (mg/mL): 3

Kinetic Assay Data

**slope of A88E A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	2.96E-01	3.04E-01	3.02E-01
B	25.00	2.19E-01	2.42E-01	2.27E-01
C	8.33	1.89E-01	1.99E-01	2.24E-01
D	2.78	1.21E-01	1.23E-01	1.61E-01
E	0.93	5.20E-02	2.75E-02	4.50E-02
F	0.31	2.00E-02	1.15E-02	1.70E-02
G	0.10	7.00E-03	5.00E-03	6.50E-03
H	0.03	1.00E-03	1.00E-03	1.00E-03

Yield (mg/mL): 3 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 21:58:11.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.00E-01	7.42E-01	8.28E-01
B	25.00	6.01E-01	6.25E-01	6.55E-01
C	8.33	5.00E-01	4.69E-01	5.05E-01
D	2.78	3.21E-01	3.51E-01	3.74E-01
E	0.93	1.48E-01	1.40E-01	1.62E-01
F	0.31	5.70E-02	5.80E-02	6.75E-02
G	0.10	2.10E-02	2.05E-02	2.20E-02
H	0.03	1.00E-03		1.00E-03

Yield (mg/mL): 7.569 Dilution Factor: 100×

Protein purified on 4/11/2023 and assayed on 4/13/2023.
Data uploaded by mspiridonova and last updated on 2024-09-29 19:16:59.

Design Data

Rosetta Score for A88E: -1.443

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.