D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant T218Q

Expression Data

Yield (mg/mL): 4.4

Kinetic Assay Data

**slope of T218Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.4754	0.4808	0.4828
B	25.00	0.3886	0.3925	0.371
C	8.33	0.2915	0.2581	0.2738
D	2.78	0.1797	0.1779	0.1902
E	0.93	0.0924	0.08705	0.09231
F	0.31	0.0355	0.02825	0.03815
G	0.10	0.01225	0.01005	0.01355
H	0.03	0.00025	0.0003	0.0002

Yield (mg/mL): 4.4 Dilution Factor: 100×

Protein purified on 11/8/2023 and assayed on 11/15/2023.
Data uploaded by ttylam and last updated on 2024-10-07 14:26:44.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.3829	0.4375	0.4025
B	25.00	0.3373	0.3516	0.3016
C	8.33	0.2148	0.2543	0.2200
D	2.78	0.1821	0.1617	0.1675
E	0.93	0.06415	0.08460	0.08690
F	0.31	0.02565	0.03030	0.03640
G	0.10	0.009350	0.01060	0.01040
H	0.03		0.0003000	0.0003000

Yield (mg/mL): 4.3 Dilution Factor: 100×

Protein purified on 9/10/2024 and assayed on 9/10/2024.
Data uploaded by ttylam and last updated on 2024-10-07 13:56:17.

Design Data

Rosetta Score for T218Q: 22.755

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.