D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H178F

Expression Data

Yield (mg/mL): 0.435

Kinetic Assay Data

**slope of H178F A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0360	0.0328	0.0304
B	25.00	0.0331	0.0320	0.0315
C	8.33	0.0302	0.0250	0.0242
D	2.78	0.0163	0.0148	0.0147
E	0.93	0.00760	0.00695	0.00700
F	0.31	0.00340	0.00315	0.00320
G	0.10	0.001250	0.001150	0.001300
H	0.03	0.000300	0.000300

Yield (mg/mL): 0.435 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 21:08:02.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1193	0.1123	0.1084
B	25.00	0.1097	0.0945	0.0935
C	8.33	0.0857	0.0748	0.0739
D	2.78	0.0490	0.0457	0.0472
E	0.93	0.02215	0.02030	0.02075
F	0.31	0.00840	0.00820	0.00815
G	0.10	0.003250	0.003200	0.003150
H	0.03	0.000150	0.000300	0.000200

Yield (mg/mL): 1.517 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 20:00:54.

Design Data

Rosetta Score for H178F: -3.457

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.