D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant Q19T

Expression Data

Yield (mg/mL): 0.889

Kinetic Assay Data

**slope of Q19T A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.0647	0.0582	0.0579
B	25.00	0.0553	0.0495	0.0514
C	8.33	0.04	0.0376	0.0374
D	2.78	0.0261	0.022	0.0221
E	0.93	0.0118	0.0092	0.0105
F	0.31	0.00485	0.0045	0.00425
G	0.10	0.0017	0.0016	0.0019
H	0.03	0.00025		0.00025

Yield (mg/mL): 0.889 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 21:21:38.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1193	0.1123	0.1084
B	25.00	0.1097	0.0945	0.0935
C	8.33	0.0857	0.0748	0.0739
D	2.78	0.0490	0.0457	0.0472
E	0.93	0.02215	0.02030	0.02075
F	0.31	0.00840	0.00820	0.00815
G	0.10	0.003250	0.003200	0.003150
H	0.03	0.000150	0.000300	0.000200

Yield (mg/mL): 1.517 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by jmpender and last updated on 2024-10-22 20:00:54.

Design Data

Rosetta Score for Q19T: -0.223

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.