D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant H315P

Expression Data

Yield (mg/mL): 1.231

Kinetic Assay Data

**slope of H315P A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.09365	0.07870	0.07815
B	25.00	0.08125	0.06635	0.05965
C	8.33	0.05880	0.05025	0.06165
D	2.78	0.05070	0.04000	0.03625
E	0.93		0.02560	0.02430
F	0.31		0.01390	0.01435
G	0.10	0.01095	0.005050	0.007300
H	0.03	0	0	0

Yield (mg/mL): 1.231 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by Wteavir and last updated on 2024-10-23 13:43:33.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	0.1215	0.1022	0.1065
B	25.00	0.1007	0.07705	0.08220
C	8.33	0.07390	0.06290	0.06355
D	2.78	0.05295	0.05725	0.05875
E	0.93	0.02680	0.03725	0.03790
F	0.31		0.01915	0.02005
G	0.10		0.007800	0.008150
H	0.03	0.0002500	0.0001000	0.0002000

Yield (mg/mL): 1.585 Dilution Factor: 100×

Protein purified on 10/10/24 and assayed on 10/14/24.
Data uploaded by Wteavir and last updated on 2024-10-23 12:49:55.

Design Data

Rosetta Score for H315P: -1.348

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.