D2D CURE | Data | Raw Characterization Data

Raw Data for BglB Variant D25Q

Expression Data

Yield (mg/mL): 5.72

Kinetic Assay Data

**slope of D25Q A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	8.09E-01	5.47E-01	4.46E-01
B	25.00	5.49E-01	4.26E-01	4.50E-01
C	8.33	4.06E-01	3.27E-01	3.34E-01
D	2.78	2.95E-01	2.32E-01	2.25E-01
E	0.93	1.42E-01	1.10E-01	1.09E-01
F	0.31	6.34E-02	5.70E-02	5.12E-02
G	0.10	3.32E-02	2.53E-02	2.18E-02
H	0.03	3.00E-04		2.50E-04

Yield (mg/mL): 5.72 Dilution Factor: 100×

Protein purified on 9/18/2024 and assayed on 9/19/2024.
Data uploaded by vrshaank and last updated on 2024-10-24 14:13:58.

Reference Data for WT

**slope of WT A_{420 nm} (min⁻¹)**
	[S] (mm)	1	2	3
A	75.00	4.32E-01	3.75E-01	3.72E-01
B	25.00	3.41E-01	2.56E-01	2.59E-01
C	8.33	2.51E-01	1.98E-01	2.32E-01
D	2.78	1.92E-01	1.48E-01	1.38E-01
E	0.93		7.96E-02	7.22E-02
F	0.31	5.27E-02	4.50E-02	3.66E-02
G	0.10	2.41E-02	1.98E-02	1.98E-02
H	0.03	4.50E-04	5.50E-04	3.00E-04

Yield (mg/mL): 3.647 Dilution Factor: 100×

Protein purified on 9/18/2024 and assayed on 9/19/2024.
Data uploaded by vrshaank and last updated on 2024-10-24 10:56:17.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Justin B. Siegel: <jbsiegel@ucdavis.edu>.