Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	5.99E-01	5.93E-01	6.85E-01
	B	25.00	4.90E-01	5.58E-01	5.92E-01
	C	8.33	4.43E-01	4.73E-01	4.36E-01
	D	2.78	2.74E-01	3.11E-01	2.38E-01
	E	0.93	1.01E-01	1.06E-01	1.13E-01
	F	0.31	3.54E-02	4.41E-02	4.00E-02
	G	0.10	1.39E-02	1.72E-02	1.47E-02
	H	0.03	2.00E-04	2.50E-04

Yield (mg/mL): 6.462     Dilution Factor:  100×

Download these data

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:29:47.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.50E-04	6.50E-04	8.00E-04
	B	48.3	3.00E-04	3.00E-04	3.50E-04
	C	45.7	9.50E-04	9.00E-04	9.50E-04
	D	42.4	6.20E-02	7.41E-02	7.36E-02
	E	37.7	3.77E-01	3.66E-01	3.73E-01
	F	33.6	4.14E-01	4.10E-01	4.06E-01
	G	31.3	4.03E-01	3.87E-01	4.10E-01
	H	30.0	4.03E-01	4.11E-01	3.41E-01

Download these data

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:43:08.

Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.