Raw Data for BglB Variant W120S

Expression Data

 0.384

Kinetic Assay Data

slope of W120S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 8.40E-03 8.50E-03
B 25.00 3.80E-03 3.80E-03
C 8.33 1.75E-03 1.95E-03 1.20E-03
D 2.78 9.50E-04 7.50E-04 4.50E-04
E 0.93 5.50E-04 7.00E-04 5.00E-04
F 0.31 5.00E-04 0 2.00E-04
G 0.10 1.50E-04 1.50E-04 2.00E-04
H 0.03 0 2.50E-04 1.50E-04
 0.384      10×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:49:43.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.99E-01 5.93E-01 6.85E-01
B 25.00 4.90E-01 5.58E-01 5.92E-01
C 8.33 4.43E-01 4.73E-01 4.36E-01
D 2.78 2.74E-01 3.11E-01 2.38E-01
E 0.93 1.01E-01 1.06E-01 1.13E-01
F 0.31 3.54E-02 4.41E-02 4.00E-02
G 0.10 1.39E-02 1.72E-02 1.47E-02
H 0.03 2.00E-04 2.50E-04
 6.462      100×

Protein purified on 10/22/2024 and assayed on 10/29/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:29:47.


Thermostability Assay Data

slope of W120S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0 0
B 48.3 0 0
C 45.7 3.00E-04 2.50E-04
D 42.4 2.50E-04 3.00E-04 2.50E-04
E 37.7 6.00E-04 5.50E-04
F 33.6 7.50E-04 6.00E-04 6.50E-04
G 31.3 7.00E-04 6.50E-04 5.50E-04
H 30.0 3.50E-04 7.00E-04 8.50E-04

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 15:50:58.


Reference Data for WT

slope of W120S A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.50E-04 6.50E-04 8.00E-04
B 48.3 3.00E-04 3.00E-04 3.50E-04
C 45.7 9.50E-04 9.00E-04 9.50E-04
D 42.4 6.20E-02 7.41E-02 7.36E-02
E 37.7 3.77E-01 3.66E-01 3.73E-01
F 33.6 4.14E-01 4.10E-01 4.06E-01
G 31.3 4.03E-01 3.87E-01 4.10E-01
H 30.0 4.03E-01 4.11E-01 3.41E-01

Protein purified on 10/22/2024 and assayed on 11/5/2024.
Data uploaded by srumjahn and last updated on 2024-11-16 14:43:08.


Citations

The unpublished data above were collected by students from Truckee Meadows Community College. For inquiries, please contact Sharif M Rumjahn: <srumjahn@tmcc.edu>.