Raw Data for BglB Variant N163S

Expression Data

SDS-PAGE
 0.302

Kinetic Assay Data

slope of N163S A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.1546 0.1526 0.1566
B 25.00 0.1566 0.1468 0.1342
C 8.33 0.1342 0.099 0.0958
D 2.78 0.0851 0.0742 0.0844
E 0.93 0.0371 0.0354
F 0.31 0.0328 0.0295 0.0319
G 0.10 0.014 0.0132 0.015
H 0.03 0.0072 0.0073 0.0073
 0.302      100×

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by aguiri01 and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00
B 25.00 0.0958 0.0874
C 8.33 0.073 0.0675
D 2.78 0.0535 0.0476
E 0.93 0.0206 0.0189
F 0.31 0.0185 0.0142
G 0.10 0.008 0.0044
H 0.03 0.0041 0.0033
 0.834      100×

Protein purified on 4/20/21 and assayed on 4/20/21.
Data uploaded by aguiri01 and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N163S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.8397 1.041
B 31.8
C 33.6
D 35.5 0.8737 0.7404
E 37.3 0.6084 0.2833
F 39.1 0.8082 0.5741
G 40.9 0.1698 0.1704
H 42.7 0.0072 0.0058
I 44.5 0.0004 0.0008
J 46.4 0.0002 0.0002
K 48.1 0.0002 0.0001
L 50.0 0 0

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by aguiri01 and last updated on 2022-07-28 15:51:56.


Reference Data for WT

slope of N163S A420 nm (min−1)
T (°C) 1 2
A 30.0 0.6649
B 31.8 0.7928
C 33.6 0.7785
D 35.5 0.6394
E 37.3 0.7241
F 39.1 0.6772
G 40.9 0.2588
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 16:00:19.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.