Raw Data for BglB Variant N220F

Expression Data

SDS-PAGE
 0.713

Kinetic Assay Data

slope of N220F A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 9.82E-02 1.16E-01 1.34E-01
B 25.00 1.16E-01 1.14E-01 1.24E-01
C 8.33 7.80E-02 6.90E-02 8.72E-02
D 2.78 5.40E-02 4.37E-02 5.04E-02
E 0.93 2.50E-02 1.90E-02 2.20E-02
F 0.31 9.10E-03 7.70E-03 1.08E-02
G 0.10 2.80E-03 2.20E-03 3.70E-03
H 0.03 8.00E-04 7.00E-04
 0.713      100×

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by XandraN220F and last updated on 2022-06-27 07:04:03.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.132 0.1296
B 25.00 0.1202 0.1221
C 8.33 0.0805 0.0851
D 2.78 0.0499 0.0523
E 0.93 0.0228 0.0224
F 0.31 0.0085 0.0084
G 0.10 0.0037 0.0034
H 0.03 0.0014 0.0014
 0.834      100×

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-06-27 07:04:03.


Thermostability Assay Data

slope of N220F A420 nm (min−1)
T (°C) 1 2
A 30.0 5.64E-01 6.00E-01
B 31.8
C 33.6
D 35.5 0.7708 0.3719
E 37.3 0.5539 0.6637
F 39.1 0.6047 0.3861
G 40.9 0.1149 0.1347
H 42.7 0.0056 0.0068
I 44.5 0.0004 0.0003
J 46.4 0.0003 0.0002
K 48.1 0.0003 0.0001
L 50.0 0.0002 0.00007

Protein purified on 4/21/21 and assayed on 4/28/21.
Data uploaded by XandraN220F and last updated on 2022-07-28 15:06:09.


Reference Data for WT

slope of N220F A420 nm (min−1)
T (°C) 1 2
A 30.0 0.6649
B 31.8 0.7928
C 33.6 0.7785
D 35.5 0.6394
E 37.3 0.7241
F 39.1 0.6772
G 40.9 0.2588
H 42.7 0.0309
I 44.5 0.0012
J 46.4 0.0004
K 48.1 0.0003
L 50.0 0.0002

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 16:00:19.


Design Data

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.