Raw Data for Wild-Type BglB

Expression Data

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	0.132	0.1296
	B	25.00	0.1202	0.1221
	C	8.33	0.0805	0.0851
	D	2.78	0.0499	0.0523
	E	0.93	0.0228	0.0224
	F	0.31	0.0085	0.0084
	G	0.10	0.0037	0.0034
	H	0.03	0.0014	0.0014

Yield (mg/mL): 0.834     Dilution Factor:  100×

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Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-06-27 07:04:03.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2
	A	30.0	0.6649
	B	31.8	0.7928
	C	33.6	0.7785
	D	35.5	0.6394
	E	37.3	0.7241
	F	39.1	0.6772
	G	40.9	0.2588
	H	42.7	0.0309
	I	44.5	0.0012
	J	46.4	0.0004
	K	48.1	0.0003
	L	50.0	0.0002

Download these data

Protein purified on 4/20/2021 and assayed on 5/4/2021.
Data uploaded by TadenW and last updated on 2022-07-28 16:00:19.

Citations

The unpublished data above were collected by students from Gettysburg College. For inquiries, please contact Jason W. Labonte: <jlabonte@ndm.edu>.