Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 0.164

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	4.56E-03	4.10E-03	3.50E-03
	B	25.00	2.09E-03	1.98E-03	2.08E-03
	C	8.33	1.29E-03	1.20E-03	1.25E-03
	D	2.78	7.42E-04	6.90E-04	6.93E-04
	E	0.93	3.48E-04	3.62E-04	3.72E-04
	F	0.31	1.63E-04	1.43E-04	1.73E-04
	G	0.10	8.99E-05	9.31E-05	9.21E-05
	H	0.03	3.46E-05	3.13E-05	3.19E-05

Yield (mg/mL): 0.164     Dilution Factor:  100×

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Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:54:06.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.63E-05	4.17E-05	5.31E-05
	B	48.3	3.04E-05	4.94E-05	6.00E-05
	C	45.7	4.79E-05	4.91E-05
	D	42.4	3.17E-04	3.32E-04	3.36E-04
	E	37.7	8.69E-04	8.07E-04	7.46E-04
	F	33.6	7.14E-04	8.26E-04	8.72E-04
	G	31.3	1.22E-03	1.16E-03	1.05E-03
	H	30.0	1.13E-03	1.14E-03	1.13E-03

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Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 12:01:34.

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.