Raw Data for BglB Variant A88E
Expression Data
0.46
Kinetic Assay Data
| [S] (mm) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 75.00 | 9.57E-04 | 9.41E-04 | 9.00E-04 | ||
| B | 25.00 | 6.92E-04 | 7.46E-04 | 7.41E-04 | ||
| C | 8.33 | |||||
| D | 2.78 | 3.11E-04 | 2.99E-04 | 3.83E-04 | ||
| E | 0.93 | 1.91E-04 | 1.96E-04 | 2.05E-04 | ||
| F | 0.31 | 9.10E-05 | 8.93E-05 | 1.02E-04 | ||
| G | 0.10 | 6.63E-05 | 6.94E-05 | 5.94E-05 | ||
| H | 0.03 | 4.56E-05 | 6.23E-05 | 3.16E-05 |
Protein purified on 10/14/2024 and assayed on 10/21/2024.
Data uploaded by apereira4 and last updated on 2024-11-18 13:06:16.
Reference Data for WT
Wild-type data not found. It may have been rejected in the curation process and deleted from the database.
Thermostability Assay Data
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 3.23E-05 | 4.10E-05 | 2.81E-05 | ||
| B | 48.3 | 2.57E-05 | 3.13E-05 | 2.60E-05 | ||
| C | 45.7 | 2.79E-05 | 2.47E-05 | |||
| D | 42.4 | 1.26E-04 | 1.42E-04 | 1.26E-04 | ||
| E | 37.7 | 3.00E-04 | 2.88E-04 | 3.27E-04 | ||
| F | 33.6 | 3.47E-04 | 3.45E-04 | 3.52E-04 | ||
| G | 31.3 | 3.64E-04 | 3.75E-04 | 3.63E-04 | ||
| H | 30.0 | 3.72E-04 | 3.83E-04 | 3.94E-04 |
Reference Data for WT
| T (°C) | 1 | 2 | 3 | |||
|---|---|---|---|---|---|---|
| A | 50.0 | 2.57E-05 | 2.66E-05 | |||
| B | 48.3 | 2.46E-05 | 2.31E-05 | |||
| C | 45.7 | 4.04E-05 | 4.34E-05 | |||
| D | 42.4 | 3.14E-04 | 3.25E-04 | 3.12E-04 | ||
| E | 37.7 | 6.66E-04 | 5.57E-04 | 6.69E-04 | ||
| F | 33.6 | 7.47E-04 | 7.14E-04 | 7.36E-04 | ||
| G | 31.3 | 7.41E-04 | 7.58E-04 | |||
| H | 30.0 | 7.98E-04 | 7.96E-04 | 8.43E-04 |
Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 11:59:59.
Design Data
-1.442
Citations
The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.