Raw Data for BglB Variant E406R

Expression Data

Yield (mg/mL): 0.147

Thermostability Assay Data

slope of E406R A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.64E-05	3.83E-05	3.09E-05
	B	48.3	5.86E-05	2.11E-05	3.61E-05
	C	45.7	1.34E-05	3.34E-05	2.61E-05
	D	42.4	1.24E-05	3.83E-05	1.90E-05
	E	37.7	3.19E-05	1.33E-05	1.99E-05
	F	33.6	3.86E-05	3.10E-05	4.13E-05
	G	31.3	5.09E-05	4.70E-05
	H	30.0	4.53E-05		4.03E-05

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Protein purified on 9/14/24 and assayed on 10/28/24.
Data uploaded by adavila1 and last updated on 2024-11-20 22:00:56.

Reference Data for WT

slope of E406R A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0	4.63E-05	4.17E-05	5.31E-05
	B	48.3	3.04E-05	4.94E-05	6.00E-05
	C	45.7	4.79E-05	4.91E-05
	D	42.4	3.17E-04	3.32E-04	3.36E-04
	E	37.7	8.69E-04	8.07E-04	7.46E-04
	F	33.6	7.14E-04	8.26E-04	8.72E-04
	G	31.3	1.22E-03	1.16E-03	1.05E-03
	H	30.0	1.13E-03	1.14E-03	1.13E-03

Download these data

Protein purified on 10/14/2024 and assayed on 10/28/2024.
Data uploaded by enielse and last updated on 2024-11-18 12:01:34.

Design Data

Citations

The unpublished data above were collected by students from Loyola University Chicago. For inquiries, please contact Emma Feeney: <enielse@luc.edu>.