Raw Data for Wild-Type BglB

Expression Data

Yield (mg/mL): 1.28

Kinetic Assay Data

slope of WT A_{420 nm} (min⁻¹)

		[S] (mm)	1	2	3
	A	75.00	3.39E-01	3.33E-01	3.31E-01
	B	25.00	2.81E-01
	C	8.33		2.81E-01	2.87E-01
	D	2.78	2.01E-01	2.68E-01	1.93E-01
	E	0.93	9.25E-02	9.43E-02	9.43E-02
	F	0.31	3.19E-02	4.15E-02	3.19E-02
	G	0.10	1.25E-02	1.27E-02	1.34E-02
	H	0.03	0

Yield (mg/mL): 1.28     Dilution Factor:  100×

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Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by vgutta and last updated on 2024-12-20 18:30:29.

Thermostability Assay Data

slope of WT A_{420 nm} (min⁻¹)

		T (°C)	1	2	3
	A	50.0			0
	B	48.3	5.00E-04	5.00E-04	5.00E-04
	C	45.7	1.10E-03	1.10E-03
	D	42.4	9.70E-02	8.64E-02	8.24E-02
	E	37.7	4.62E-01	4.81E-01	3.93E-01
	F	33.6	4.48E-01	4.32E-01	4.42E-01
	G	31.3	4.58E-01	4.52E-01	4.39E-01
	H	30.0	4.46E-01	4.49E-01	4.59E-01

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Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by vgutta and last updated on 2024-11-20 12:30:52.

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.