Raw Data for BglB Variant N421D

Expression Data

SDS-PAGE
 0.57

Kinetic Assay Data

slope of N421D A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.2461 0.2464 0.2359
B 25.00 0.2047 0.2049
C 8.33 0.1605 0.1713 0.1668
D 2.78 0.0995 0.0951 0.1011
E 0.93 0.0432 0.05415 0.05195
F 0.31 0.0260 0.02425 0.02570
G 0.10 0.0090 0.009800 0.010500
H 0.03 0.0014 0
 0.57      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by lexiehalliwell and last updated on 2024-11-20 11:51:31.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 0.5247 0.5256 0.5216
B 25.00 0.4892 0.4143 0.4612
C 8.33 0.3967 0.3530 0.3534
D 2.78 0.2508 0.2718 0.2323
E 0.93 0.11700 0.11980 0.13790
F 0.31 0.05700 0.05310
G 0.10 0.022600 0.017800 0.029250
H 0.03 0.001600 0.001400 0.001750
 1.35      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 11:44:07.


Thermostability Assay Data

slope of N421D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0 0.0008000 0.000500
B 48.3 0.000700 0.0007000
C 45.7 0.001200 0.001300 0.001000
D 42.4 0.06950 0.05890 0.07190
E 37.7 0.1774 0.1878 0.1864
F 33.6 0.1804 0.1771 0.1791
G 31.3 0.1756 0.1850 0.1803
H 30.0 0.1919 0.1894 0.1768

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by lexiehalliwell and last updated on 2024-11-20 11:55:00.


Reference Data for WT

slope of N421D A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0007 0.0006 0.0007
B 48.3 0.0009 0.0008 0.0010
C 45.7 0.0009 0.0008 0.0008
D 42.4 0.0550 0.0482 0.0703
E 37.7 0.43300 0.39770 0.42040
F 33.6 0.46650 0.42670 0.43730
G 31.3 0.472900 0.503300 0.463300
H 30.0 0.467600 0.495700 0.482000

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by johnnyc14 and last updated on 2024-11-20 12:07:31.


Design Data

-0.191

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.