Raw Data for BglB Variant L269V

Expression Data

SDS-PAGE
 0.63

Kinetic Assay Data

slope of L269V A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 2.06E-01 1.53E-01 1.65E-01
B 25.00 1.29E-01 1.22E-01 1.28E-01
C 8.33 1.08E-01 1.08E-01 1.02E-01
D 2.78 8.34E-02 6.81E-02 7.84E-02
E 0.93 3.73E-02 2.83E-02 3.32E-02
F 0.31 1.40E-02 1.10E-02 1.29E-02
G 0.10 4.40E-03 4.00E-03 4.45E-03
H 0.03 0 0
 0.63      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by jkdhill and last updated on 2024-11-20 12:14:20.


Reference Data for WT

slope of WT A420 nm (min−1)
[S] (mm) 1 2 3
A 75.00 5.28E-01 5.14E-01 5.20E-01
B 25.00 4.31E-01 4.06E-01 4.27E-01
C 8.33 3.86E-01 3.60E-01 3.65E-01
D 2.78 2.23E-01 2.25E-01 2.29E-01
E 0.93 1.04E-01 1.02E-01 1.05E-01
F 0.31 3.98E-02 3.95E-02 4.15E-02
G 0.10 1.39E-02 1.41E-02 1.30E-02
H 0.03 7.00E-04 8.50E-04
 1.59      100×

Protein purified on 10/23/2024 and assayed on 10/30/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:36:41.


Thermostability Assay Data

slope of L269V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 4.00E-04 6.00E-04 8.00E-04
B 48.3 6.00E-04 0 1.20E-03
C 45.7 8.00E-04 9.00E-04
D 42.4 3.25E-02 3.94E-02 3.66E-02
E 37.7 1.10E-01 1.15E-01 1.23E-01
F 33.6 1.16E-01 1.30E-01 1.19E-01
G 31.3 1.32E-01 1.33E-01 1.34E-01
H 30.0 0 0

Protein purified on 10/23/2024 and assayed on 11/6/2024.
Data uploaded by jkdhill and last updated on 2024-11-20 12:42:02.


Reference Data for WT

slope of L269V A420 nm (min−1)
T (°C) 1 2 3
A 50.0 0.0012000 0.000600
B 48.3 0.0011 0.0009000
C 45.7 0.0011 0.001100 0.001200
D 42.4 0.0791 0.09140 0.11770
E 37.7 0.6272 0.5683 0.5244
F 33.6 0.6226 0.6248 0.6226
G 31.3 0.6319 0.6288 0.6255
H 30.0 0.628 0.6750 0.6181

Protein purified on 11/6/2024 and assayed on 11/6/2024.
Data uploaded by ASDl and last updated on 2024-11-20 12:53:49.


Design Data

21.647

Citations

The unpublished data above were collected by students from University of California, Davis. For inquiries, please contact Ashley Vater: <awvater@ucdavis.edu>.